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分子别名（Synonym）
RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF
表达区间及表达系统（Source）
Human VEGF110 Protein, His Tag (VE0-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 136 (Accession # NP_001165097.1).
Predicted N-terminus: His
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 14.6 kDa. The protein migrates as 15 kDa and 18-20 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Human VEGF110 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human VEGF110 Protein, His Tag (Cat. No. VE0-H52H3) is more than 85% and the molecular weight of this protein is around 30-38 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human VEGFR1/R2, Fc Tag at 5 μg/mL (100 μL/well) can bind Human VEGF110 Protein, His Tag (Cat. No. VE0-H52H3) with a linear range of 1-78 ng/mL (QC tested).
Protocol
Immobilized Human VEGF110 Protein, His Tag (Cat. No. VE0-H52H3) at 5 μg/mL (100 μL/well) can bind Anti-VEGF(Avastin), Human IgG1 with a linear range of 0.2-2 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Human VEGFR1/R2 captured on Protein A Chip can bind Human VEGF110 Protein, His Tag (Cat. No. VE0-H52H3) with an affinity constant of 13.8 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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