Example of cross-reactivity: According to the Claudin-18.2 immunohistochemical cross-reactivity experiment, there is no cross-reactive staining result between Claudin-18.2 and Claudin-18.1.
> Use Claudin-18.2 primary antibody (Cat. No.
HCS-S278) to stain normal gastric tissue, strong positive staining of gastric gland cell cytoplasm and/or cell membrane, negative staining of other tissue cells.
Human Stomach Tissue, 4X
Human Stomach Tissue, 20X
> Normal lung tissue is stained with Claudin-18.2 primary antibody (Cat. No.
HCS-S278), and lung tissue cells are negatively stained (Claudin-18.2 normal lung 20X).
Human Lung Tissue, 4X
Human Lung Tissue, 20X
* The human gene Claudin-18 has two protein isoforms, Claudin-18.1 and Claudin-18.2, which differ within the N-terminal 69 amino acids. Claudin-18.1 expression is restricted to the lungs, whereas Claudin-18.2 expression is restricted to the stomach.
Staining example of human tumor tissue samples: use Claudin-18.2 primary antibody (Cat. No.
HCS-S278) to stain gastric cancer, colorectal cancer，pancreatic cancer, and ovarian cancer tissue samples respectively
Human Gastric Cancer, 20X
Human Colorectal Cancer, 20X
Human Pancreatic Cancer, 20X
Human Ovarian Cancer, 20X
> Using Claudin-18.2 primary antibody (Cat. No.
HCS-S278) to stain human tumor tissue samples, positive cell cytoplasm and/or cell membrane specific positive staining (staining intensity ≥ 1), no non-specific staining (staining intensity < 1) , no background staining (staining intensity < 1 ).
Staining example of applicable platform: use Claudin-18.2 primary antibody (Cat. No.
HCS-S278) to stain normal gastric tissues at Leica BOND-III and Dako link 48.
Leica BOND-III-10X
Leica BOND-III-40X
Dako link 48-10X
Dako link 48-40X
> Using Claudin-18.2 primary antibody (Cat. No.
HCS-S278) to stain normal gastric tissues, positive cell cytoplasm and/or cell membrane specific positive staining (staining intensity ≥ 1), no non-specific staining (staining intensity < 1) , no background staining (staining intensity < 1 ).
Performance comparison
Titer: ACRO at 1/1000 dilution and Competitor A at 1/200 dilution, color intensity is similar
Claudin18.2 (ACRO)
1:1000-40X
Claudin18.2 (Competitor A)
1:200-40X
Claudin18.2 (Competitor A)
1:500-40X
> Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Claudin-18.2 with ACRO/Competitor A. Membranous staining on human gastric epithelial cells is observed. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Sensitivity: ACRO staining intensity is stronger than Competitor A
Claudin18.2 (ACRO)
1:1000-4X
Claudin18.2 (Competitor A)
1:200-4X
Claudin18.2 (Competitor A)
1:500-4X
Claudin18.2 (ACRO)
1:1000-40X
Claudin18.2 (Competitor A)
1:200-40X
Claudin18.2 (Competitor A)
1:500-40X
> Immunohistochemical analysis of paraffin-embedded human gastric cancer labeling Claudin-18.2 with ACRO/Competitor A. Membranous staining on tumor cells is observed. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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