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- ProductSizeAmountActiveMax® DLL4 μBeads, premium grade (for cells, 20 μm)2.5 mg1.5 × 10⁶ beadsActiveMax® DLL4 μBeads, premium grade (for cells, 20 μm)10 mg (2.5 mg × 4)6.0 × 10⁶ beads
背景（Background）
ActiveMax® Human DLL4 μBeads, premium grade (for cells, 20μm) are produced under sterile manufacturing conditions (ISO 5) , and no animal- or human-derived components are used throughout the production process. It is produced under our rigorous quality control system that includes a comprehensive set of tests including sterility and endotoxin tests.
表达区间及表达系统（Source）
ActiveMax® Human DLL4 μBeads, premium grade (for cells, 20 μm) are uniform, superparamagnetic beads of 20 µm with streptavidin on its surface and coupled with biotinylated Human DLL4 Protein, expressed from human 293 cells (HEK293) and contains AA Ser 27 - Pro 524 (Accession # NP_061947.1 ).
应用说明（Application）
ActiveMax® Human DLL4 μBeads, premium grade (for cells, 20 μm) is designed to synergistically activate Notch signaling in hematopoietic stem/progenitor cells (HSPCs). The synergistic interactions between Notch ligand Delta-like 4 and VCAM-1 are leveraged to enhance Notch signaling to support efficient T-cell lineage commitment, so the beads is recommended to use for in vitro production of induced pluripotent stem cell derived T cells.
* We also carry ActiveMax® Streptavidin μBeads, premium grade (for cells)(Cat. No. MBS-C009), and it can be used for convenient preparation of your own sterile protein/antibody coupled beads for cell-based assay, cell sorting, and other applications. The ActiveMax® Streptavidin μBeads, premium grade can also be used together with other ActiveMax® μBeads, premium grade as Isotype control.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
This product is stable in storage under the following conditions: -20˚C for 12 months in lyophilized state. -70°C for 3 months under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles after reconstitution. Immediate use after reconstitution is highly recommended.
无菌（Sterility）
Negative
内毒素（Endotoxin）
Less than 0.04 EU per μg by the LAL method.
注意事项（Important Note）
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
质量管理控制体系（QMS）
产品展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
Assay of human DLL4 protein on the μBeads surface by Flow cytometry. Add 100ul of 1:80 Anti-DLL4 Antibody/PE-anti-Mouse IgG1 dilution into 5e5 of ActiveMax® Human DLL4 μBeads, premium grade (for cells, 20 μm)(Cat. No. MBS-C024), negative control as well. PE signals was used to evaluate DLL4 beads activity (QC tested).
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