产品详情
优势与特点(FAB)
- High Sensitivity - Detects dsRNA at as low as 8.988 pg/mL, crucial for trace dsRNA detection in mRNA vaccine QC.
- High Specificity - Specifically identifies dsRNA, not cross - reacting with ssRNA or dsDNA for accurate detection.
- Broad Applicability - Recognizes >40bp dsRNA, including various modified types, suitable for diverse scenarios.
- Flexible usage - Customers can purchase 5-OMe-UTP dsRNA, N1-Me-pUTP dsRNA, and pUTP dsRNA standard according to their own needs.
- Good Precision and Accuracy - High intra-assay as well as inter-assay precision, and accurate recovery for high, middle, low-concentration samples to ensure reliable results.
产品参数(Product Specifications)
Assay TypeSandwich-ELISAAnalytedsRNAFormat96T(8×12 strips)Reactivity/Regulatory StatusRUOSensitivity8.988 pg/mLStandard Curve Range25 pg/mL-800 pg/mLAssay Time2 hr 20 minSuitable Sample TypeFor the detection and quantitative determination of dsRNA in samplesSample volume100 uL产品概述(Product Overview)
Double-Stranded RNA (dsRNA) ELISA Kit is based on the Sandwich-ELISA method and is used for the rapid detection and quantitative determination of dsRNA concentrations in mRNA vaccine formulations. It is designed to precisely detect and control the amount of dsRNA in mRNA vaccines, which is crucial for ensuring vaccine quality and safety.
应用说明(Application)
1.mRNA Vaccine Development
2.Quality Control of IVT Products
3.Research on Viral Infections
It is for research use only.
重构方法(Reconstitution)
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储(Storage)
2-8℃
组分(Materials Provided)
IDComponentsSizeRES092-C01Pre-coated Anti-dsRNA Monoclonal Antibody (K1) Microplate1 plate(8×12 strips)RES092-C02Unmodified dsRNA (400bp) Standard10 μg×2RES092-C03HRP-Anti-dsRNA Monoclonal Antibody (J2)10 μg×2RES092-C0410×Washing Buffer50 mLRES092-C05Sample Dilution Buffer50 mLRES092-C06HRP-Antibody (J2) Dilution Buffer25 mLRES092-C07Substrate Solution12 mLRES092-C08Stop Solution7 mL质量管理控制体系(QMS)
产品展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
批内差异(Intra-Assay Statistics)
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
批间差异(Inter-Assay Statistics)
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
回收率(Recovery)
Three samples with different concentrations were tested to calculate the recovery rate.
专属性(Specificity)
The kit has been tested to specifically identify dsRNA, and do not identify ssRNA , dsDNA.
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