resDetect™ Kanamycin ELISA Kit

产品详情

Assay Type	Competitive-ELISA
Analyte	Kanamycin
Format	96T(8×12 strips)
Regulatory Status	RUO
Sensitivity	0.5 ng/mL
Standard Curve Range	0.5 ng/mL-40.5 ng/mL
Assay Time	1 hr 20 min
Suitable Sample Type	For the detection and quantitative determination of kanamycin residues in plasmid DNA raw materials for cell and gene therapy.
Sample volume	50 μL

应用说明（Application）
The Kanamycin ELISA Kit is calibrated against the NIFDC standard.
The kit is developed for the detection and quantitative determination of kanamycin residues in plasmid DNA raw materials and proteins for CGT, vaccine and other biological drugs.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
2-8℃

原理（Assay Principles）
The Kanamycin quantitative detection kit adopts the indirect competitive ELISA method, and the pre-coated conjugated kanamycin antigen on the microstrip competes with the residual kanamycin in the sample to bind the enzyme-labeled anti-kanamycin monoclonal antibody, and then uses a microplate reader to detect the absorbance value by adding TMB substrate, and the absorbance value is negatively correlated with the content of kanamycin in the sample. The kit takes only about one hour and 20 minutes to operate and has a linear range of 0.5 ng/mL to 40.5 ng/mL.
质量管理控制体系（QMS）

典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

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