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组分（Materials Provided）
Box A(2-8°C)IDComponentsSizeRIPO-RWM010-C01Liver organoid Basal medium MM225mlBox B(-20°C)IDComponentsSizeRIPO-RWM010-1-C01Liver organoid Supplement MM-120mlRIPO-RWM010-1-C02Liver organoid Supplement MM-25ml产品描述（Product Details）
Liver organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative to the human liver. Human iPSC-Derived Liver Organoid Maintenance Kit allows long-term maturation and maintenance of the liver organoids.
产品特征（Product Specification）
The basic medium of this kit is a serum-free, well-defined medium with minimal batch variation to which differentiation factors are added. This medium does not contain antibiotics, the addition of which may affect organoid differentiation.
存储（Storage）
The unopened Box A is stable for 12 months from the date of manufacture if stored under 2-8°C.
The unopened Box B is stable for 12 months from the date of manufacture if stored under -20°C.
The opened kit should be stored according to components table.- *请注意，为了支持完整的类器官培养和维持过程，本产品必须与Human iPSC-Derived Liver Organoid Expansion and Maintenance Medium(Cat. No RIPO-RWM010）一起使用。您可以点击此链接获取产品信息。
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产品示意（Product Diagram）
Protocol Diagram of liver organoid differentiation.
Orgnaoid Histology
H&E Staining of liver organoid.
Marker Expression
The liver organoids show expression of hepatocytes (HFN4a), hepatobiliary tracts (CK19), Albumin (ALB) and vascular endothelial cell (CD31).
Pre-mature liver organoids show liver lobules-like structure.
Organoid Activity
Liver organoids show better performance than HepG2 cell line in terms of the expression of Albumin, CYP3A4, CYP1A2, CYP2C9, CYP7A1 and BSEP.
Liver organoid show response to Rifampicin treatment as indication by the increase fluorescence intensity of CYP3A4.
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