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产品参数（Product Specifications）
Assay TypeFRETAnalyteDNase IFormat96T/480TRegulatory StatusRUOSensitivity3.9×10⁻⁵ UStandard Curve Range0.0025 U-3.9×10⁻⁵ UAssay Time30 minSuitable Sample TypeFor the quantitative determination of DNase I in the environment, some biological materials, common molecular biological reagents such as reaction buffers.Sample volume10 μL & 80 μL产品概述（Product Overview）
This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]
应用说明（Application）
The DNase Activity Assay Kit (Fluorescence) is a convenient and sensitive assay tool to test the presence of DNase in buffers, reagents, and other components.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
-20℃
组分（Materials Provided）
ItemsSize （96tests）Size（480tests）DNase Substrate2 nmol10 nmol10X Reaction Buffer for DNase10 mL10 mLDNase I (1U/μL)20 μL50 μLTE Buffer (pH 8.0)1.5 mL6 mLNuclease-free Water10 mL50 mL原理（Assay Principles）
The DNase Activity Assay Kit (Fluorescence) is based on a fluorophore-labeled DNase substrate. When the sample does not contain DNase activity, the substrate is stable and does not produce a fluorescent signal; when the sample contains DNase activity, the substrate is degraded, resulting in a gradual enhanced fluorescence signal, the rate of increase in fluorescence signal is positively correlated with the number and activity of enzymes. Use a fluorescence microplate reader to measure at the wavelength of ex/em = 535/565 nm to determine whether the sample is contaminated by DNase.
质量管理控制体系（QMS）
产品展示
活性（Bioactivity）-Fluorescence
Please refer to DS document for the assay protocol.
Add 90 μL of the working DNase Substrate solution to each 96-well plate, and add 10 μL of DNase I standards (0.00000390625-0.00025 U/μL*10μL/well = 0.0000390625-0.0025U/well), incubate the plate in the fluorometer (BMG CLARIOstar) collecting real-time data at 1 minute intervals for 30 minutes at 37°C using the settings described in this section. The DNase Activity Assay can be evaluated in rigorous kinetic terms using real-time data.
This assay kit employs a standard detection of DNase I. Add 90 μL working DNase Substrate solution to each 96-well plate, and add 10 μL DNase I standards ((0.00000390625-0.00025 U/μL*10μL/well = 0.0000390625-0.0025U/well), incubate for 30 minutes at 37°C. Then measure the fluorescence using the settings described in this section in a fluorometer (BMG CLARIOstar). Take RFU of standards as the ordinate and DNase concentration as the abscissa. Four parameters logistic are used to draw the standard curve (QC tested).
批内差异（Intra-Assay Statistics）
Eight replicates of each of seven samples containing different concentrations of DNase were tested in one assay, Intra-Assay Precision CV＜10%.
批间差异（Inter-Assay Statistics）
Eight replicates of each of seven samples containing different concentrations of DNase were tested in eight independent assays, Inter-Assay Precision CV<15%.
稳定性 (Stability)
The probe is freeze-thawed 3 times, the kit performance meets the requirements, the sensitivity is not reduced, and the CV< 10%.
回收率（Recovery）
Three different concentrations of DNase was spiked into four different kinds of matrixes. The range of the recovery rate is 80%~120%.
干扰效应(Interference effect)
DNase Activity Assay Kit (Fluorescence) (Cat. No. ASE-A002) and RNase Activity Assay Kit (Fluorescence) (Cat. No. ASE-A001) were used to detect nuclease residues in the same sample. No significant cross-reactivity or interference was observed.
- *Complete Your Research: RNase Activity Assay Kit (Fluorescence) (ACROBiosystems, cat#ASE-A001) is prepared.
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