resDetect™ HEK293 resDNA Quantitation Kit (qPCR)

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OPA-R006-100tests
$2,000.00
In Stock - Delivery in 2-3 business days
OPA-R006-500tests (100tests X 5)
$9,000.00
OPA-R006
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Product Overview

HEK293 resDNA Quantitation Kit is designed for quantitative detection of residual HEK293 DNA in biopharmaceutical productions (Antibodies, cells, viruses, etc.). Based on real-time quantitative PCR (qPCR) method, this kit makes detection the residual DNA form HEK293 cells rapid and reliable. The lower limit of quantitation is 30 fg/µL. All procedures are typically in less than 4 hours.

Use the kit after you extract host cell DNA from test samples. For achieving the better DNA recovery, it is recommended to use the resDNA Sample Preparation Kit Ⅱ (Magnetic Beads) (Cat. No. OPA-R024) in combination.

Features
  • High sensitivity for optimal product safety: LLOD (15 fg/µL) for detection of residue host cell DNA
  • High specificity: No cross-reactivity with unrelated DNA
  • Validation: ICH Q2(R2) as validation of analytical procedures
  • High-quality: This kit is manufactured in ISO 13485 standard facility.
Application

The kit is used for quantitative detection of residual HEK293 DNA in biopharmaceutical production.

For use in quality control/manufacturing process only.

It is for research use only.

Technical Specifications
DNA Technical Specifications
Attention

If your experimental workflow involves host cell DNA sample preparation, please use the recommended sample preparation kits listed in the table. This ensures that the buffers used during both sample preparation and residual DNA quantitation remain consistent, supporting reliable and accurate results.

DNA Attention
Materials Provided
ID
Components
Size
OPA-R006-01
2×qPCR Master Mix
1.0 mL×2
OPA-R006-02
HEK293 Primer & Probe Mix
700 μL
OPA-R006-03
HEK293 DNA Control(3ng/μL)
100 μL
OPA-R006-04
Dilution Buffer
1.5 mL×3
OPA-R006-05
DNase/RNase-Free Water
1.0 mL