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resDetect™ BSA ELISA Kit

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RES-A001-96tests
¥2940.00
合计0件 产品金额¥ 0

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  • 优势与特点(FAB)

    1. Rapid - Results can be obtained within 90 minutes.
    2. Reference Standard Traceability - The reference standard is traceable to the National Standard NIFDC (cat # 140619).
    3. Rigorous Method Validation - Validation reports compliant with ICH guidelines can be provided upon request.
    4. High Sensitivity - Sensitivity for Bovine Serum Albumin (BSA) is < 0.5 ng/mL.
    5. Strong Specificity - No cross-reactivity with non-target proteins.
    6. Excellent Buffer Compatibility - Compatible with a wide range of buffer systems.
  • 产品参数(Product Specifications)

    Assay Type
    Sandwich-ELISA
    Analyte
    BSA (Bovine serum albumin)
    Format
    96T(8×12 strips)
    Regulatory Status
    RUO
    Sensitivity
    <0.5ng/mL
    Standard Curve Range
    0.5ng/mL-40.5ng/mL
    Assay Time
    1.5 hr
    Suitable Sample Type
    For the quantitative determination of BSA (Bovine serum albumin)
    Sample volume
    50μL

  • 产品概述(Product Overview)

    resDetect™ BSA ELISA Kit is based on the ELISA sandwich method and is used to detect and quantitatively determine Bovine Serum Albumin (BSA) residues in biological products. This kit is designed to support optimal purification process development and routine quality control of in-process streams and final products in various biotechnological processes, such as cell and tissue culture. The standard BSA has been calibrated against the NIFDC standard (cat # 140619) to ensure accurate assay results. It can also be used as a universal detection tool for the quantitative determination of BSA.

  • 存储(Storage)

    2-8℃

  • 组分(Materials Provided)

    ID
    Components
    Size
    RES01-C01
    Pre-Coated Anti-BSA Antibody Microplate
    1 plate(8×12 strips)
    RES01-C02A
    BSA (Bovine serum albumin) Standard (200ng/mL)
    500 μL
    RES01-C02B
    BSA (Bovine serum albumin) Standard 5 (40.5ng/mL)
    500 μL
    RES01-C02C
    BSA (Bovine serum albumin) Standard 4 (13.5ng/mL)
    500 μL
    RES01-C02D
    BSA (Bovine serum albumin) Standard 3 (4.5ng/mL)
    500 μL
    RES01-C02E
    BSA (Bovine serum albumin) Standard 2 (1.5ng/mL)
    500 μL
    RES01-C02F
    BSA (Bovine serum albumin) Standard 1 (0.5ng/mL)
    500 μL
    RES01-C02G
    BSA (Bovine serum albumin) Standard 0 (0ng/mL)
    500 μL
    RES01-C03
    HRP-Anti-BSA Antibody
    100 μL
    RES01-C04
    1×Sample Dilution Buffer
    50 mLX2
    RES01-C05
    20×Washing Buffer
    25 mL
    RES01-C06
    Substrate Solution
    12 mL
    RES01-C07
    Stop Solution
    8 mL

  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

产品展示

  • 典型数据-Typical Data

    Please refer to DS document for the assay protocol.

     BSA TYPICAL DATA

    Detection of BSA ( Bovine serum albumin) by ELISA Assay.
    Immobilized Anti-BSA Antibody can bind BSA (Bovine serum albumin) Detection was performed using HRP-Anti- BSA Antibody with sensitivity of 0.5 ng/mL (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

验证
  • 批内差异(Intra-Assay Statistics)

    Three samples of known concentration were tested ten times on one plate to assess intra-assay precision , Intra-Assay Precision CV≤15%.

     BSA INTRA-ASSAY STATISTICS
  • 批间差异(Inter-Assay Statistics)

    Three samples of known concentration were tested in ten separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.

     BSA INTER-ASSAY STATISTICS
  • 准确度(Accuracy)

    Five samples of known concentration were tested ten times to assess Accuracy , Accuracy recovery rate 80-120%.

     BSA ACCURACY
  • 干扰效应(Interference effect)

    We have conducted interference effect test about frequently-used buffers, they have excellent buffer compatibility. For specific buffers, it is recommended that you verify recovery to determine the minimum dilution ratio.

     BSA INTERFERENCE EFFECT
  • 专属性(Specificity)

    High, medium and low concentrations of BSA were added to Human serum (v/v 1%), Horse serum (v/v 10%) , Human Serum Albumin (1.25 mg/mL) and Rabbit serum (v/v 10%), the recovery rate of BSA was used as the specific validation index.

     BSA SPECIFICITY

    High, medium and low concentrations of BSA were added to MDCK( 2×106 cells/mL), HEK293( 3.5×106 cells/mL) , CHO( 2×106 cells/mL) and T-lymphocyte( 2.15×106 cells/mL) the recovery rate of BSA was used as the specific validation index.

     BSA SPECIFICITY

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