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分子别名（Synonym）
FOLH1,PSMA,GIG27,FOLH,NAALAD1,PSM,NAALADase I,GCPII,FGCP
表达区间及表达系统（Source）
Biotinylated Human PSMA, His,Avitag, premium grade (PSA-H82Qb) is expressed from human 293 cells (HEK293). It contains AA Lys 44 - Ala 750 (Accession # Q04609-1).
Predicted N-terminus: His
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 83.1 kDa. The protein migrates as 95-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Biotin
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 25 mM MES, 500 mM NaCl, pH6.5 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 6 months under sterile conditions.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Biotinylated Human PSMA, His,Avitag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human PSMA, His,Avitag, premium grade (Cat. No. PSA-H82Qb) is more than 85% and the molecular weight of this protein is around 190-225 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-FACS
2e5 of PSMA-CAR-293 cells transfected with anti-PSMA-scFv were stained with 100 μL of 1 μg/mL of Biotinylated Human PSMA, His,Avitag, premium grade (Cat. No. PSA-H82Qb) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routiney tested).
Protocol
活性（Bioactivity）-ELISA
Immobilized Monoclonal Anti-Human PSMA Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human PSMA, His,Avitag, premium grade (Cat. No. PSA-H82Qb) with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
活性（Bioactivity）
Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is >200 pmol/min/μg, as measured under the described conditions (QC tested).
Protocol
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