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HRSV (B) Post-fusion glycoprotein F0, His Tag (MALS verified)

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货号-规格
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Qty.
RSF-V52H9-50ug
¥4725.00
RSF-V52H9-500ug
¥29400.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    Postfusion glycoprotein F0/post-F protein (RSV)

  • 表达区间及表达系统(Source)

    HRSV (B) Post-fusion glycoprotein F0, His Tag (RSF-V52H9) is expressed from human 293 cells (HEK293).

  • 蛋白结构(Molecular Characterization)

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 55.2 kDa. The protein migrates as 16-18 kDa and 43-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    Unconjugated

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, 150 mM NaCl, pH5.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

数据展示

  • 电泳(SDS-PAGE)

    Postfusion glycoprotein F0/post-F protein (RSV) SDS-PAGE

    HRSV (B) Post-fusion glycoprotein F0, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Postfusion glycoprotein F0/post-F protein (RSV) SEC-MALS

    The purity of HRSV (B) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H9) is more than 90% and the molecular weight of this protein is around 185-215 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     Postfusion glycoprotein F0/post-F protein (RSV) ELISA

    Immobilized HRSV (B) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H9) at 1 μg/mL (100 μL/well) can bind Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) with a linear range of 0.1-4 ng/mL and not bind Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (D25) (QC tested).

    Protocol

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背景介绍

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. The RSV fusion glycoprotein (RSV F) is the principal target of RSV neutralizing antibodies in human sera. The RSV F is a type I viral fusion protein synthesized as inactive, single-chain polypeptides that assemble into trimers. RSV F fuses the viral and host cell membranes by irreversible protein refolding from the labile prefusion conformation to the stable post-fusion conformation. Both states exhibit epitopes targeted by neutralizing antibodies, and post-fusion RSV F is being developed as a vaccine candidate.

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