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分子别名（Synonym）
IL33,DV27,C9ORF26,IL1F11,NFHEV,DKFZp586H0523,DVS27,NFEHEV,RP11-575C20.2
表达区间及表达系统（Source）
Human IL-33 Protein, His Tag (IL3-H52H7) is expressed from human 293 cells (HEK293). It contains AA His 109 - Thr 270 (Accession # O95760-1).
Predicted N-terminus: His
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 20.2 kDa. The protein migrates as 25-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Human IL-33 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-33 Protein, His Tag (Cat. No. IL3-H52H7) was greater than 85% as determined by SEC-HPLC.
活性（Bioactivity）-ELISA
Immobilized Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) at 5 μg/mL (100 μL/well) can bind Human IL-33 Protein, His Tag (Cat. No. IL3-H52H7) with a linear range of 5-40 ng/mL (QC tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) on Protein A Biosensor, can bind with Human IL-33 Protein, His Tag (Cat. No. IL3-H52H7) an affinity constant of 1.31 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
活性（Bioactivity）-CELL BASE
Human IL-33 Protein, His Tag (Cat. No. IL3-H52H7) induces IL-8 secretion in HUVECs. The EC50 for this effect is 193.0-311.8 ng/mL (Routinely tested).
Protocol
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