产品详情
分子别名(Synonym)
IL-2 R beta & IL-2 R alpha & IL-2 R gamma
表达区间及表达系统(Source)
Biotinylated Human IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag (ILG-H82F4) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 & Glu 22 - Ser 212 & Leu 23 - Asn 254 (Accession # P14784-1 (IL-2RB) & P01589-1 (IL-2RA) & P31785-1 (IL-2RG)).
Predicted N-terminus: Ala 27 & Glu 22
Request for sequence蛋白结构(Molecular Characterization)
Biotinylated Human IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag is produced by co-expression of IL-2RB and IL-2RA and IL-2RG, has a calculated MW of 52.8 kDa (IL-2RB) and 77.1 kDa (IL-2RA&IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™) and subunit IL-2RA&IL-2RG is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The reducing (R) protein migrates as 65-70 kDa and 115-120 kDa respectively due to glycosylation.
偶联(Conjugate)
Biotin
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
数据展示
电泳(SDS-PAGE)
Biotinylated Human IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%undefined.
SEC-MALS
The purity of Biotinylated Human IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag (Cat. No. ILG-H82F4) is more than 85% and the molecular weight of this protein is around 180-200 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Human IL-2, Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2 RA & IL-2 RB& IL-2 RG, Fc,Avitag&Fc,Avitag (Cat. No. ILG-H82F4) with a linear range of 2-78 ng/mL (QC tested).
ProtocolImmobilized Human IL-15, premium grade (Cat. No. IL5-H4117) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2 RA & IL-2 RB& IL-2 RG, Fc,Avitag&Fc,Avitag (Cat. No. ILG-H82F4) with a linear range of 0.039-1.25 μg/mL (Routinely tested).
Protocol
活性(Bioactivity)-BLI
Loaded Biotinylated Human IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag (Cat. No. ILG-H82F4) on SA Biosensor, can bind Human IL-2, His Tag (Cat. No. IL2-H52H8) with an affinity constant of 2.41 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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