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分子别名（Synonym）
Envelope glycoprotein gp41/gp41 (HIV)
表达区间及表达系统（Source）
HIV-1 (HXB2) GP41 Pre-hairpin intermediate Protein, His Tag (GP1-H51H3) is expressed from E. coli cells. It contains AA Ala 533 - Leu 856 (Accession # P04578).
Predicted N-terminus: Met
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 44.3 kDa. The protein migrates as Band dispersion when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>85% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
HIV-1 (HXB2) GP41 Pre-hairpin intermediate Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized HIV-1 (HXB2) GP41 Pre-hairpin intermediate Protein, His Tag (Cat. No. GP1-H51H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HIV-1 GP41 Antibody, Human IgG1 (2F5) with a linear range of 0.2-16 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Monoclonal Anti-HIV-1 GP41 Antibody, Human IgG1 (2F5) captured on Protein A Chip can bind HIV-1 (HXB2) GP41 Pre-hairpin intermediate Protein, His Tag (Cat. No. GP1-H51H3) with an affinity constant of 0.468 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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