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分子别名（Synonym）
CLDN4,claudin 4
表达区间及表达系统（Source）
Human Claudin-4 Protein, His,Twin-Strep Tag (CL4-H5587) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Val 209 (Accession # O14493-1).
Predicted N-terminus: Met
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus and a twin strep tag at the C-terminus.
The protein has a calculated MW of 27.5 kDa. The protein migrates as 25-27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 12 months under sterile conditions.
质量管理控制体系（QMS）
- *The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.
数据展示
电泳（SDS-PAGE）
Human Claudin-4 Protein, His,Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human Claudin-4 Protein, His,Twin-Strep Tag (Cat. No. CL4-H5587) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Claudin 4 antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Human Anti-CLDN4 antibody-xi5A5, Human IgG1 captured on Protein A Chip can bind Human Claudin-4 Protein, His,Twin-Strep Tag (Cat. No. CL4-H5587) with an affinity constant of 2.77 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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