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分子别名（Synonym）
ACVR2B,ACTRIIB,MGC116908
表达区间及表达系统（Source）
Biotinylated Human Activin RIIB Protein, His,Avitag (ACB-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Thr 137 (Accession # Q13705-1 ).
Predicted N-terminus: Ser 19
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 17.2 kDa. The protein migrates as 25-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Biotin
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Biotinylated Human Activin RIIB Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human Latent Activin A, His Tag (Cat. No. ACA-H424x) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Activin RIIB Protein, His,Avitag (Cat. No. ACB-H82E3) with a linear range of 0.005-0.313 μg/mL (QC tested).
Protocol
Immobilized Human Activin A Protein, Tag Free (Cat. No. ACA-H421b) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Activin RIIB Protein, His,Avitag (Cat. No. ACB-H82E3) with a linear range of 0.3-5 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Biotinylated Human Activin RIIB Protein, His,Avitag (Cat. No. ACB-H82E3) immobilized on CM5 Chip can bind Human Latent Activin A, His Tag (Cat. No. ACA-H424x) with an affinity constant of 0.147 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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