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Mouse Anti-SARS-CoV-2 (XBB.1) Antibody IgG Titer Serologic Assay Kit (Spike Trimer)

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RAS-T148-96tests
¥6825.00
合计0件 产品金额¥ 0

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  • 组分(Materials Provided)

    ID
    Components
    Size
    RAS148-C01
    Pre-coated SARS-CoV-2 Spike Trimer (XBB.1) Microplate
    1 plate
    RAS148-C02
    SARS-CoV-2 Antibody Positive Control
    100 μL
    RAS148-C03
    SARS-CoV-2 Antibody Negative Control
    100 μL
    RAS148-C04
    HRP-Conjugated Antibody
    50 μL
    RAS148-C05
    10 x Washing Buffer
    50 mL
    RAS148-C06
    Dilution Buffer
    50 mL
    RAS148-C07
    Substrate Solution
    12 mL
    RAS148-C08
    Stop Solution
    7 mL

  • 产品概述(Product Overview)

    Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide.As the virus spreads globally, the continuous emergence of new mutant strains escalated the challenge on humans.To facilitate the mutant-related research, drug trials and vaccine development, a high-throughput assay to measure IgG antibodies against the mutants is in urgent need.

  • 应用说明(Application)

    The kit is developed for titer measurement of Anti-SARS-CoV-2 (XBB.1) IgG antibody (Spike Trimer) in mouse serum.

    It is for research use only.

  • 存储(Storage)

    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • 原理(Assay Principles)

    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Mouse serum by SARS-CoV-2 Spike Trimer. The Kit consists of Pre-coated with SARS-CoV-2 Spike Trimer (XBB.1) Microplate,Positive Control,Negative Control and HRP-conjugated antibody.

    Your experiment will include 4 simple steps:

    a) The samples and Control are diluted by Dilution Buffer.Add your sample to the plate.

    b) Add the HRP-conjugated antibody diluted by Dilution Buffer to the plate.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

数据展示

  • 典型数据-Typical Data

    Please refer to DS document for the assay protocol.

     Spike Trimer TYPICAL DATA

    For determination of antibody titer: the positive sample was diluted with a gradient, and the antibody titer of the sample corresponds to the highest dilution factor that still yields a positive reading.

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