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ClinMax™ Human IL-17A / CTLA8 ELISA Kit

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货号-规格
价格
Qty.
CEA-C092-96tests
¥2940.00
CEA-C092-480tests (96tests X 5)
¥ 13230.00
 ¥11907.00
合计0件 产品金额¥ 0

产品详情

  • 产品概述(Product Details)

    ClinMax™ Human IL-17A / CTLA8 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IL-17A / CTLA8 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:

    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

  • 应用说明(Application)

    The kit is developed for quantitative detection of natural and recombinant human IL-17A / CTLA8 in serum, plasma and cell culture supernatants.

    It is for research use only.

  • 流程图(Workflow)

    IL-17A Workflow

  • 关键信息(Key Features)

    Assay Type
    Sandwich-ELISA
    Analyte
    IL-17A/CTLA8
    Format
    96-wells plate breakable into 12 x 8 wells strips
    Reactivity
    Human
    Sensitivity
    <6pg/mL
    Assay Time
    2hr
    Sample volume
    50 μL
    Range
    31.25 pg/mL-2000 pg/ml
    Sample Type
    Cell Culture Supernatants, Plasma, Serum.

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • 存储(Storage)

    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • 组分(Materials Provided)

    ID
    Components
    Size
    CEA092-C01
    Pre-coated Anti-IL-17A Antibody Microplate
    1 plate
    CEA092-C02
    Human IL-17A Standard
    20μg ×2
    CEA092-C03
    Biotin-Anti-IL-17A Antibody Con. Solution
    300 μL
    CEA092-C04
    Biotin-Antibody Dilution Buffer
    8 mL
    CEA092-C05
    Streptavidin-HRP Con. Solution
    500 uL
    CEA092-C06
    Streptavidin-HRP Dilution Buffer
    15 mL
    CEA092-C07
    20× Washing Buffer
    50 mL
    CEA092-C08
    Sample Dilution Buffer
    15 mL ×2
    CEA092-C09
    Substrate Solution
    12 mL
    CEA092-C10
    Stop Solution
    6 mL
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

数据展示

  • 典型数据-Typical Data

    Please refer to DS document for the assay protocol.

     IL-17A TYPICAL DATA

    For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IL-17A is less than 6 pg/mL.

验证
  • 基质效应(Matrix Effect)

    3 types of materials were tested to observe if there were matrix effect (interference). If the concentration of hemoglobin (simulated hemolysis) is less than 3500 mg/dL(2%), the concentration of triglyceride (simulated lipid blood) is less than 3 mg/mL, testing results will not be affected.

     IL-17A MATRIX EFFECT
  • 稀释线性(Dilution Linearity)

    High concentrations of human IL-17A serum samples were diluted with 1:2, 1:4, 1:8 and 1:16 ratios for gradient dilution to evaluate the linearity of the assay. In the serum samples, the average detection rate of IL-17A was 90.14%.

     IL-17A DILUTION LINEARITY
  • 批内差异(Intra-Assay Statistics)

    Ten replicates of each of 4 samples containing different IL-17A concentrations were tested in one assay. Acceptable criteria: CV<10%.

     IL-17A INTRA-ASSAY STATISTICS
  • 批间差异(Inter-Assay Statistics)

    Five samples containing different concentrations of IL-17A were tested in independent assays. Acceptable criteria: CV<15%.

     IL-17A INTER-ASSAY STATISTICS
  • 回收率(Recovery)

    Recombinant IL-17A was spiked into 3 human serum samples, and then analyzed. The average recovery of IL-17A for serum samples is 85.37%.

     IL-17A RECOVERY

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前沿进展

 
药物研发进展
  • 英文全称:

    Interleukin 17A

  • 中文全称:

    白细胞介素-17A

  • 种类:

  • 上市药物数量:

    5 详情

  • 临床药物数量:

    28 详情

  • 最高研发阶段:

    批准上市

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