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抗体来源（Source）
The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Spike RBD. The antibody is specific against the Beta (B.1.351) and Gamma (P.1) variant of SARS-CoV-2, and has no binding with the spike RBD of the wild type virus, the Alpha (B.1.1.7) variant, the Delta (B.1.617.2) variant and the Omicron (B.1.1.529, BA.2) variant.
克隆号（Clone）
AS106
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Hybridoma Monoclonal
特异性（Specificity）
This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. No cross-reactivity is detected with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.
应用（Application）
ApplicationRecommended UsageELISA0.1-12.5 ng/mL纯度（Purity）
95% as determined by SDS-PAGE.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS106) (Beta & Gamma Specific) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized SARS-CoV-2 spike protein (Beta, Cat. No. SPN-C52Hk and Gamma, Cat. No. SPN-C52Hg) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS106) (Beta & Gamma Specific) (Cat. No. SPD-M416) with a linear range of 6-8 ng/mL. The antibody does not bind spike protein of WT (Cat. No. SPN-C52H7), Alpha (Cat. No. SPN-C52H6) and Delta (Cat. No. SPN-C52He) (QC tested).
Protocol
Immobilized SARS-CoV-2 Spike RBD protein B.1.1.529/Omicron (Cat. No. SPD-C522e), BA.2/Omicron ( Cat. No. SPD-C522g), BA.2.12.1/Omicron (Cat. No. SPD-C522q) and BA.3/Omicron (Cat. No. SPD-C522i) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS106) (Beta & Gamma Specific) (Cat. No. SPD-M416) with a linear range of 0.05-1.56 ng/mL. The antibody does not bind Spike RBD protein BA.4&BA.5/Omicron (Cat. No. SPD-C522r), Spike protein BA.4/Omicron (Cat. No. SPN-C5229) and BA.5/Omicron (Cat. No. SPN-C522e) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS106) (Beta & Gamma Specific) (Cat. No. SPD-M416) loaded on AMC Biosensor can bind SARS-CoV-2 S protein, His Tag (Beta, Cat. No. SPN-C52Hk) with an affinity constant of 19.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS106) (Beta & Gamma Specific) (Cat. No. SPD-M416) loaded on AMC Biosensor can bind SARS-CoV-2 S protein, His Tag (Gamma, Cat. No. SPN-C52Hg) with an affinity constant of 20.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS106) (Beta & Gamma Specific) (Cat. No. SPD-M416) loaded on AMC Biosensor does not bind SARS-CoV-2 S protein, His Tag (WT, Cat. No. SPN-C52H7) as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS106) (Beta & Gamma Specific) (Cat. No. SPD-M416) loaded on AMC Biosensor does not bind SARS-CoV-2 S protein, His Tag (Alpha, Cat. No. SPN-C52H6) as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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