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Recombinant Streptavidin Protein (MALS verified)

COAMS
DS
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STN-N5116-5mg (1mg X 5)
¥2205.00
合计0件 产品金额¥ 0
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产品详情

  • 分子别名（Synonym）

    Streptavidin,SA

  • 表达区间及表达系统（Source）

    Recombinant Streptavidin (STN-N5116) is expressed from E.coli cells.

    Predicted N-terminus: Met

  • 蛋白结构（Molecular Characterization）

    This protein carries no "tag". The protein has a calculated MW of 13.8 kDa.

  • 偶联（Conjugate）

    Unconjugated

  • 应用说明（Application）

    ELISA assay

  • 内毒素（Endotoxin）

    Less than 1.0 EU per μg by the LAL method.

  • 纯度（Purity）

    95% as determined by SDS-PAGE.

    95% as determined by SEC-MALS.

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in NaCl, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 2 years in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

产品展示

  • 电泳（SDS-PAGE）

    Streptavidin SDS-PAGE

    The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%undefined.

  • SEC-MALS

    Streptavidin SEC-MALS

    The purity of Recombinant Streptavidin (Cat. No. STN-N5116) is more than 95% and the molecular weight of this protein is around 54-67 kDa verified by SEC-MALS.

    Report

  • 活性（Bioactivity）-ELISA

    Streptavidin ELISA

    Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a with a linear range of 0.1-6 ng/mL (QC tested).

    Protocol

  • Streptavidin ELISA

    Immobilized Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (Routinely tested).

    Protocol

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背景介绍

Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI).Streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting.

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