产品详情
分子别名(Synonym)
Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD
表达区间及表达系统(Source)
SARS-CoV-2 Spike RBD, His Tag (BA.4&BA.5/Omicron) (SPD-C522r) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H)). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.4 and BA.5; GISAID clade: GRA).
Predicted N-terminus: Arg 319
Request for sequence蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.7 kDa. The protein migrates as 33-36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联(Conjugate)
Unconjugated
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
产品展示
电泳(SDS-PAGE)
SARS-CoV-2 Spike RBD, His Tag (BA.4&BA.5/Omicron) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of SARS-CoV-2 Spike RBD, His Tag (BA.4&BA.5/Omicron) (Cat. No. SPD-C522r) is more than 85% and the molecular weight of this protein is around 34-44 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD, His Tag (BA.4&BA.5/Omicron) (Cat. No. SPD-C522r) with a linear range of 16-125 ng/mL (QC tested).
ProtocolImmobilized SARS-CoV-2 Spike RBD, His Tag (BA.4&BA.5/Omicron) (Cat. No. SPD-C522r) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1 (Cat. No. SPD-M265) with a linear range of 0.1-6 ng/mL (QC tested).
ProtocolImmobilized SARS-CoV-2 Spike RBD, His Tag (BA.4&BA.5/Omicron) (Cat. No. SPD-C522r) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M130) with a linear range of 0.4-13 ng/mL (Routinely tested).
Protocol
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