关注公众号
收藏产品 产品详情
分子别名（Synonym）
CD33,SIGLEC3,gp67
表达区间及表达系统（Source）
Human Siglec-3, Fc Tag (CD3-H5257) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1).
Predicted N-terminus: Asp 18
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 52.9 kDa. The protein migrates as 64-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human Siglec-3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human Siglec-3, Fc Tag (Cat. No. CD3-H5257) is more than 90% and the molecular weight of this protein is around 115-145 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human Siglec-3 Antibody at 1 μg/mL (100 μL/well) can bind Human Siglec-3, Fc Tag (Cat. No. CD3-H5257) with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
活性（Bioactivity）-BLI
Loaded Siglec-3 MAb (Mouse IgG1) on AMC Biosensor, can bind Human Siglec-3, Fc Tag (Cat. No. CD3-H5257) with an affinity constant of 0.329 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
用户评价 发表评论
药物研发进展 
联系我们 
项目合作