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Human LIGHT / TNFSF14 Protein, Fc Tag, active trimer (MALS verified)

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LIT-H5269-100ug
¥3675.00
LIT-H5269-1mg (500ug X 2)
¥24255.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    CD258 antigen,CD258,HVEM-L,LIGHT,LTg,TNFSF14,TR2,HVEML

  • 表达区间及表达系统(Source)

    Human LIGHT Protein, Fc Tag (LIT-H5269) is expressed from human 293 cells (HEK293). It contains AA Asp 74 - Val 240 (Accession # O43557-1) trimer Design.

    Predicted N-terminus: Pro

  • 蛋白结构(Molecular Characterization)

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 82.6 kDa. The protein migrates as 116 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    Unconjugated

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    90% as determined by SDS-PAGE.

    90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    LIGHT SDS-PAGE

    Human LIGHT Protein, Fc Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    LIGHT SEC-MALS

    The purity of Human LIGHT Protein, Fc Tag (Cat. No. LIT-H5269) is more than 90% and the molecular weight of this protein is around 180-200 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     LIGHT ELISA

    Immobilized Human HVEM, His Tag (Cat. No. HVM-H52E9) at 5 μg/mL (100 μL/well) can bind Human LIGHT Protein, Fc Tag (Cat. No. LIT-H5269) with a linear range of 0.2-4 ng/mL (QC tested).

    Protocol
  • 活性(Bioactivity)-CELL BASE

     LIGHT CELL

    Human LIGHT Protein, Fc Tag (Cat. No. LIT-H5269) induced cytotoxicity in HT-29 cells. The ED50 for this effect is 1.29-2.38 ng/mL (Routinely tested).

    Protocol

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背景介绍

Tumor necrosis factor ligand superfamily member 14(LIGHT) is a tumor necrosis factor (TNF) superfamily ligand that regulates T cell immune responses by signaling through the herpes virus entry mediator (HVEM) and the lymphotoxin beta receptor (LTbetaR). LIGHT has emerged as a potent initiator of T cell co-stimulation signals effecting CTL-mediated tumor rejection, allograft rejection and graft versus host disease. Constitutive expression of LIGHT leads to tissue destruction and autoimmune-like disease syndromes.

前沿进展

 
药物研发进展
  • 英文全称:

    Tumor necrosis factor superfamily member 14

  • 中文全称:

    肿瘤坏死因子超家族成员14

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    2 详情

  • 最高研发阶段:

    临床二期

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