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分子别名（Synonym）
IL21,Za11,Interleukin-21
表达区间及表达系统（Source）
Biotinylated Human IL-21, Fc,Avitag, premium grade (IL1-H82F7) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Ser 162 (Accession # Q9HBE4-1).
Predicted N-terminus: Gln 30
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 43.6 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Biotin
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 0.02 EU per μg by the LAL method.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
纯度（Purity）
95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Biotinylated Human IL-21, Fc,Avitag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human IL-21, Fc,Avitag, premium grade (Cat. No. IL1-H82F7) is more than 85% and the molecular weight of this protein is around 100-125 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IL-21 R, Fc Tag (Cat. No. ILR-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-21, Fc,Avitag, premium grade (Cat. No. IL1-H82F7) with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Biotinylated Human IL-21, Fc,Avitag, premium grade (Cat. No. IL1-H82F7) immobilized on CM5 Chip can bind Human IL-21 R, Fc Tag (Cat. No. ILR-H5257) with an affinity constant of 0.165 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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