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Human IL-2 R beta&IL-2 R gamma Heterodimer Protein, His Tag&Twin-Strep Tag (MALS verified)

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ILG-H5283-100ug
¥3990.00
ILG-H5283-1mg (500ug X 2)
¥26670.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    IL-2 R beta & IL-2 R gamma,IL-2RB & IL-2RG

  • 表达区间及表达系统(Source)

    Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag (ILG-H5283) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (IL-2RB) & Leu 23 - Asn 254 (IL-2RG) (Accession # P14784-1 (IL-2RB) & P31785-1 (IL-2RG)).

    Predicted N-terminus: Ala 27 (IL-2RB) & Leu 23 (IL-2RG)

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    IL-2 R beta & IL-2 R gamma Structure

    Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 29.9 kDa (IL-2RB) and 34.1 kDa (IL-2RG). Subunit IL-2RB is fused with a polyhistidine tag at the C-terminus and subunit IL-2RG is fused with Twin-Strep tag at the C-terminus. The protein migrates as 40-44 kDa and 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    Unconjugated

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    95% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    IL-2 R beta & IL-2 R gamma SDS-PAGE

    Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%undefined.

  • SEC-MALS

    IL-2 R beta & IL-2 R gamma SEC-MALS

    The purity of Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag (Cat. No. ILG-H5283) is more than 85% and the molecular weight of this protein is around 90-115 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     IL-2 R beta & IL-2 R gamma ELISA

    Immobilized Human IL-2, Tag Free at 5 μg/mL (100 μL/well) can bind Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag (Cat. No. ILG-H5283) with a linear range of 20-78 ng/mL (QC tested).

    Protocol
  • 活性(Bioactivity)-BLI

     IL-2 R beta & IL-2 R gamma BLI

    Loaded Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag (Cat. No. ILG-H5283) on NTA Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 1.42 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol

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背景介绍

Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

前沿进展

 
药物研发进展
  • 英文全称:

    Interleukin-2 receptor subunit gamma

  • 中文全称:

    白介素-2受体γ亚基

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    13 详情

  • 最高研发阶段:

    临床二期

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