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分子别名（Synonym）
IL13RA2,CD213A2,CT19,IL-13R,IL13BP
表达区间及表达系统（Source）
Human IL-13 R alpha 2, His Tag (IL2-H52H5) is expressed from human 293 cells (HEK293). It contains AA Asp 27 - Arg 343 (Accession # Q14627-1).
Predicted N-terminus: Asp 27
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 39.0 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
90% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human IL-13 R alpha 2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human IL-13 R alpha 2, His Tag (Cat. No. IL2-H52H5) is more than 90% and the molecular weight of this protein is around 40-59 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IL-13, Fc Tag (Cat. No. IL3-H5256) at 5 μg/mL (100 μL/well) can bind Human IL-13 R alpha 2, His Tag (Cat. No. IL2-H52H5) with a linear range of 1-39 ng/mL (QC tested).
Protocol
Immobilized Human IL-13 R alpha 2, His Tag (Cat. No. IL2-H52H5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-IL-13 R alpha 2 Antibody, Mouse IgG1 with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Immobilized Monoclonal Anti-IL-13 R alpha 2 Antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Human IL-13 R alpha 2, His Tag (Cat. No. IL2-H52H5) with a linear range of 0.4-6 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human IL-13, Fc Tag (Cat. No. IL3-H5256) on Protein A Biosensor, can bind Human IL-13 R alpha 2, His Tag (Cat. No. IL2-H52H5) with an affinity constant of 6.09 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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