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Mouse IGF-I R / CD221 Protein, His Tag

COAMS
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IGR-M5223-100ug
¥3675.00
IGR-M5223-1mg (500ug X 2)
¥24255.00
合计0件 产品金额¥ 0
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  • 分子别名（Synonym）

    IGF1R,IGFR,JTK13,CD221,MGC142170,MGC142172,MGC18216

  • 表达区间及表达系统（Source）

    Mouse IGF-I R, His Tag (IGR-M5223) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - His 936 (Accession # Q60751-1).

    Predicted N-terminus: Glu 31 & Asp 742

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    IGF-I R Structure

    This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 738-RRRR-741, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.7 kDa and 24.3 kDa respectively. The protein migrates as 45-55 kDa (partial β chain), 100-125 kDa (α chain) and 150 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联（Conjugate）

    Unconjugated

  • 内毒素（Endotoxin）

    Less than 1.0 EU per μg by the LAL method.

  • 纯度（Purity）

    90% as determined by SDS-PAGE.

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

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  • 电泳（SDS-PAGE）

    IGF-I R SDS-PAGE

    Mouse IGF-I R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%undefined.

  • 活性（Bioactivity）-ELISA

    IGF-I R ELISA

    Immobilized Mouse IGF-I R, His Tag (Cat. No. IGR-M5223) at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag with a linear range of 7.8-125 ng/mL (QC tested).

    Protocol

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背景介绍

The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.

前沿进展

 
药物研发进展
  • 英文全称：

    Insulin-like growth factor I receptor

  • 中文全称：

    胰岛素样生长因子-I受体

  • 种类：

  • 上市药物数量：

    6 详情

  • 临床药物数量：

    26 详情

  • 最高研发阶段：

    批准上市

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