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HSV-2 (HG52) Glycoprotein E Protein, His Tag (MALS verified)

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GLE-H52H4-100ug
¥3990.00
GLE-H52H4-1mg (500ug X 2)
¥26670.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    Glycoprotein E/gE (HSV)

  • 表达区间及表达系统(Source)

    HSV-2 (HG52) Glycoprotein E, His Tag (GLE-H52H4) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Arg 414 (Accession # P89475).

    Predicted N-terminus: Ala 21

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    Glycoprotein E/gE (HSV) Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 45.0 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    Unconjugated

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    95% as determined by SDS-PAGE.

    90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    Glycoprotein E/gE (HSV) SDS-PAGE

    HSV-2 (HG52) Glycoprotein E, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    Glycoprotein E/gE (HSV) SEC-MALS

    The purity of HSV-2 (HG52) Glycoprotein E, His Tag (Cat. No. GLE-H52H4) is more than 90% and the molecular weight of this protein is around 50-65 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     Glycoprotein E/gE (HSV) ELISA

    Immobilized HSV-2 (HG52) Glycoprotein E, His Tag (Cat. No. GLE-H52H4) at 1 μg/mL (100 μL/well) can bind anti-HSV-2-gE antibody with a linear range of 0.2-16 ng/mL (QC tested).

    Protocol

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背景介绍

Herpesvirus infections are widely spread throughout the world population. Herpes simplex virus (HSV) belongs to the α-herpesvirus subfamily. There are two main types of HSV, HSV-1 and HSV-2, which infect humans. HSV-2 mainly causes genital lesions, whereas HSV-1 is involved in both oral and genital infections. In epithelial cells, the heterodimer gE/gI is required for the cell-to-cell spread of the virus, by sorting nascent virions to cell junctions. Once the virus reaches the cell junctions, virus particles can spread to adjacent cells extremely rapidly through interactions with cellular receptors that accumulate at these junctions. Implicated in basolateral spread in polarized cells. In neuronal cells, gE/gI is essential for the anterograde spread of the infection throughout the host nervous system.

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