选择语言

Biotinylated Human CD45 / PTPRC protein, His,Avitag™ (MALS verified)

用户评价
货号-规格
价格
Qty.
CD5-H82E7-25ug
¥3675.00
CD5-H82E7-200ug
¥14490.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    CD45,PTPRC,L-CA,T200

  • 表达区间及表达系统(Source)

    Biotinylated Human CD45, His,Avitag (CD5-H82E7) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Lys 577 (Accession # P08575-3).

    Predicted N-terminus: Gln 26

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    CD45 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 64.4 kDa. The protein migrates as 120-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    Biotin

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    95% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

产品展示

  • 电泳(SDS-PAGE)

    CD45 SDS-PAGE

    Biotinylated Human CD45, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    CD45 SEC-MALS

    The purity of Biotinylated Human CD45, His,Avitag (Cat. No. CD5-H82E7) is more than 85% and the molecular weight of this protein is around 180-210 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-BLI

     CD45 BLI

    Loaded Biotinylated Human CD45, His,Avitag (Cat. No. CD5-H82E7) on SA Biosensor, can bind Human Galectin-1, Tag Free (Cat. No. GA1-H4113) with an affinity constant of 2.0 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol

用户评价
发表评论

背景介绍

CD45 is a receptor protein tyrosine phosphatase, also known as Ly-5 or leukocyte common antigen. CD45 mainly involves in the initiation of T cell receptor signaling by controlling the activation of the Src family protein-tyrosine kinases Lck and Fyn. CD45 deficiency causes in T- and B-lymphocyte dysfunction in the form of severe combined immune deficiency. It also takes a significant role in autoimmune diseases and cancer as well as in infectious diseases including fungal infections.

前沿进展

 
药物研发进展
  • 英文全称:

    Leukocyte common antigen

  • 中文全称:

    白细胞共同抗原

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    3 详情

  • 最高研发阶段:

    临床三期

联系我们
项目合作
查看更多项目
  • 产品SKU
  • 产品详情
  • 产品展示
  • 用户评价
  • 背景介绍