选择语言

Biotinylated Human CD117 / c-kit Protein, His,Avitag (MALS verified)

用户评价
货号-规格
价格
Qty.
CD7-H82E6-25ug
¥3675.00
CD7-H82E6-200ug
¥14490.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    CD117,SCFR,c-Kit,KIT

  • 表达区间及表达系统(Source)

    Biotinylated Human CD117, His,Avitag (CD7-H82E6) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Thr 516 (Accession # P10721-2).

    Predicted N-terminus: Gln 26

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    CD117 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 58.8 kDa. The protein migrates as 70-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    Biotin

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    95% as determined by SDS-PAGE.

    90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

产品展示

  • 电泳(SDS-PAGE)

    CD117 SDS-PAGE

    Biotinylated Human CD117, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    CD117 SEC-MALS

    The purity of Biotinylated Human CD117, His,Avitag (Cat. No. CD7-H82E6) is more than 90% and the molecular weight of this protein is around 74-100 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     CD117 ELISA

    Immobilized Human SCF protein, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD117, His,Avitag (Cat. No. CD7-H82E6) with a linear range of 0.02-0.625 μg/mL (QC tested).

    Protocol

用户评价
发表评论

背景介绍

Mast/stem cell growth factor receptor Kit (c-Kit), a member of the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily, is also known as piebald trait protein (PBT), p145 c-kit, tyrosine-protein kinase Kit and CD117,which contains five Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. CD117 acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, CD117 can activate several signaling pathways. Furthermore, Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B.

前沿进展

 
药物研发进展
  • 英文全称:

    Stem cell growth factor receptor

  • 中文全称:

    干细胞生长因子受体

  • 种类:

  • 上市药物数量:

    16 详情

  • 临床药物数量:

    32 详情

  • 最高研发阶段:

    申请上市

联系我们
项目合作
查看更多项目
  • 产品SKU
  • 产品详情
  • 产品展示
  • 用户评价
  • 背景介绍