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分子别名（Synonym）
CA125,CA-125,CA125MUC-16,FLJ14303,MUC16,mucin 16,mucin-16
表达区间及表达系统（Source）
Human CA125 (13810-14451), His Tag (CA5-H52H6) is expressed from human 293 cells (HEK293). It contains AA Pro 13810 - Pro 14451 (Accession # Q8WXI7-1).
Predicted N-terminus: Pro 13810
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 73.7 kDa. The protein migrates as 90-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human CA125 (13810-14451), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CA125 (13810-14451), His Tag (Cat. No. CA5-H52H6) is more than 90% and the molecular weight of this protein is around 85-115 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human CA125 (13810-14451), His Tag (Cat. No. CA5-H52H6) at 5 μg/mL (100 μL/well) can bind Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253) with a linear range of 0.2-10 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Human Mesothelin (296-580), His Tag, premium grade (Cat. No. MSN-H522a) immobilized on CM5 Chip can bind Human CA125 (13810-14451), His Tag (Cat. No. CA5-H52H6) with an affinity constant of 1.82 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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