PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Kit

组分（Materials Provided）

应用说明（Application）

This kit is developed for screening for inhibitors of human PD-1 binding to human PD-L1.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

2-8℃

原理（Assay Principles）

This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new PD-1 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human PD-1 to immobilized human PD-L1 in a functional ELISA assay, and employs a simple colorimetric sandwich ELISA platform.

Your experiment will include 4 simple steps:

a) Coat the plate with human PD-L1.

b) Add your molecule of interest to the tests.

c) Add human PD-1-Biotin to bind the coated human PD-L1.

d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

Finally, the ability of your compound to inhibit PD-1 : PD-L1 binding will be determined by comparing OD readings among different experimental groups.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程