关注公众号
收藏产品 产品详情
产品参数（Product Specifications）
Assay TypeSandwich-ELISAAnalyteNucleaseFormat96T(8×12 strips)Reactivity/Regulatory StatusRUOSensitivity＜25pg/mLStandard Curve Range25 pg/mL-800 pg/mLAssay Time3 hr 20 minSuitable Sample TypeFor the quantitative determination the residue of nuclease in biologicals.Sample volume100 uL产品概述（Product Overview）
resDetect™ Salt Active GENIUS™ Nuclease ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine Salt Active GENIUS™ residues in biological products. It is designed to provide a reliable solution for biological product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of Salt Active GENIUS™.
应用说明（Application）
The Salt Active GENIUS™ Nuclease ELISA Kit was developed for the detection and quantitative determination of nuclease in samples from downstream processing where nuclease is used as a process or purification aid.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
2-8℃
组分（Materials Provided）
IDComponentsSizeRES054-C01Pre-coated Anti-Nuclease Antibody Microplate1 plate(8×12 strips)RES054-C02Salt Active Nuclease Standard5 μgRES054-C03Biotin-Anti-Nuclease Antibody20 μgRES054-C04Streptavidin-HRP50 μLRES054-C0510×Washing Buffer50 mLRES054-C062×Dilution Buffer50 mLRES054-C07Substrate Solution12 mLRES054-C08Stop Solution7 mL原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Nuclease. The kit consists of Pre-coated Anti-Nuclease Antibody Microplate and Nuclease Standard and Biotin-Anti-Nuclease Antibody and Streptavidin-HRP and buffers.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the Nuclease as standard. The samples and standard are diluted by Dilution Buffer.
c) Add the Biotin-Anti-Nuclease Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
质量管理控制体系（QMS）
产品展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
批内差异（Intra-Assay Statistics）
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision, Intra-Assay Precision CV≤15%.
批间差异（Inter-Assay Statistics）
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.
回收率（Recovery）
Three Salt Active Nuclease with different concentrations were tested to calculate the recovery rate.
干扰效应(Interference effect)
resDetect™ Salt Active GENIUS™ Nuclease ELISA Kit (Residue Testing) (Cat. No. RES-A054) can detect the Salt Active Nuclease in different culture supernatant with similar curve and sensitivity.
专属性（Specificity）
resDetect™ Salt Active GENIUS™ Nuclease ELISA Kit (Residue Testing) (Cat. No. RES-A054) can detect the Salt Active Nuclease from different culture supernatant and was free from the matrix effects.
用户评价 发表评论
联系我们 
项目合作