IL-4 [Biotinylated] : IL-4Rα Inhibitor Screening ELISA Kit

组分（Materials Provided）

产品概述（Product Overview）

Interleukin-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells to Th2 cells). In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine.

应用说明（Application）

This kit is developed for screening for inhibitors of human IL-4Rα binding to human IL-4.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

2-8℃

原理（Assay Principles）

This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new IL-4 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human IL-4 to immobilized human IL-4 R alpha in a functional ELISA assay, and employs a simple colorimetric sandwich ELISA platform.

Your experiment will include 4 simple steps:

a) Coat the plate with human IL-4 R alpha.

b) Add your molecule of interest to the tests.

c) Add human IL-4-Biotin to bind the coated human IL-4 R alpha.

d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

Finally, the ability of your compound to inhibit IL-4: IL-4 R alpha Rbinding will be determined by comparing OD readings among different experimental groups.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程