关注公众号
收藏产品 产品详情
抗体来源（Source）
Anti-Human IL-15 (12B11E5) mAb, Mouse IgG1 is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
克隆号（Clone）
12B11E5
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Human
免疫原（Immunogen）
Recombinant Human IL-15 is expressed from human HEK293 cells.
特异性（Specificity）
This product is a specific antibody specifically reacts with IL-15, Human. No cross-reactivity is detected with other human cytokines, including IL-2, IL-4, IL-6, IL-10, IL-21,GM-CSF,TNF-alpha and IFNγ.
应用（Application）
ApplicationRecommended UsageELISA0.2-60 ng/mL纯度（Purity）
90% as determined by SDS-PAGE.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Anti-Human IL-15 (12B11E5) mAb, Mouse IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human IL-15, His Tag (Cat. No. IL5-H52H8) at 1 μg/mL (100 μL/well) can bind Anti-Human IL-15 (12B11E5) mAb, Mouse IgG1 (Cat. No. IL5-M542) with a linear range of 0.2-4 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Anti-Human IL-15 (12B11E5) mAb, Mouse IgG1 (Cat. No. IL5-M542) captured on CM5 chip via anti-mouse antibodies surface can bind Human IL-15, His Tag (Cat. No. IL5-H52H8) with an affinity constant of 0.138 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
用户评价 发表评论
药物研发进展 
联系我们 
项目合作