GMP Human VEGF165 Protein

VEGF165​ is the predominant isoform of Vascular Endothelial Growth Factor, belonging to the Platelet-Derived Growth Factor (PDGF) family. It serves as a key cytokine regulating angiogenesis and endothelial cell function. During the differentiation of human pluripotent stem cells (hPSCs) toward endothelial cells, VEGF165 drives mesoderm cells toward endothelial progenitors and promotes endothelial cell proliferation, migration, and tube formation. In protocols for differentiation into immune cells, VEGF165 supports the construction of a vascular-like microenvironment, indirectly facilitating the homing and differentiation of hematopoietic stem/progenitor cells. It plays a synergistic role, particularly in the differentiation of myeloid immune cells such as macrophages and dendritic cells, and is often combined with cytokines like SCF, FLT3L, and IL-3 to enhance differentiation efficiency and functional maturation.

• Specific Angiogenic & Hematopoietic Driver​​: Efficiently supports hPSC-derived hematopoietic stem/progenitor cell (HSPC) expansion and endothelial cell differentiation.
• Reliable Large-Scale Supply: Ensures batch production capacity, supporting robust and consistent manufacturing scale-up.
• High Lot-to-Lot Consistency​: Ensures reliable and reproducible performance in every batch, supporting scalable applications.

• Quality Assurance: Global QMS with comprehensive and stringent QC release criteria.
• End-to-End GMP Compliance: Full manufacturing and QC under a cGMP system.
• Comprehensive Control of Adventitious Agents: Stringent biosafety from cell banks to final release.
• Comprehensive Regulatory Support: Includes RSF and DMF to meet global requirements.
• Resilient Supply Chain: Intelligent modular facilities ensure a stable global supply.
• Professional Support: Extensive manufacturing and application expertise to accelerate development.

GMP Human VEGF165 Protein (GMP-VE5H23) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4).

Less than 10 EU/mg, tested by the LAL method in compliance with USP <85> and Ph. Eur. 2.6.14.

<0.5 ng/µg of protein tested by ELISA.

<0.02 ng/μg of protein tested by qPCR.

Sterility testing was performed using the membrane filtration method in compliance with USP <71> and Ph. Eur. 2.6.1.

Negative

>95% as determined by SDS-PAGE.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with protectants.

Contact us for customized product form or formulation.

2R (13 mm neck finish)

This product is supplied and shipped on blue ice.

Upon receipt, store it immediately at -20°C or lower for long term storage.

Please avoid repeated freeze-thaw cycles.

• -20°C to -70°C for 5 years in lyophilized state;
• -70°C for 12 months under sterile conditions after reconstitution.