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分子别名（Synonym）
DDPAC,FTDP-17,MAPT,MSTD,MTBT1,Tau,PHF-tau,TAU
表达区间及表达系统（Source）
Human Tau-441, Tag Free (TAU-H5117) is expressed from E. coli cells. It contains AA Met 1 - Leu 441 (Accession # P10636-8).
Predicted N-terminus: Met 1
Request for sequence蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 45.8 kDa. The protein migrates as 30-53 kDa and 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
无菌（Sterility）
Negative
纯度（Purity）
90% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human Tau-441, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Tau-441, Tag Free (Cat. No. TAU-H5117) is more than 90% and the molecular weight of this protein is around 38-55 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ThT Assay
Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time when tau wild-type monomers (Cat. No. TAU-H5117) are combined with tau wild-type Pre-formed Fibrils (Cat. No. TAU-H5115) (Routinely tested).
Protocol
活性（Bioactivity）-ELISA
Immobilized Human Tau-441, Tag Free (Cat. No. TAU-H5117) at 1 μg/mL (100 μL/well) can bind Tau Monoclonal Antibody with a linear range of 0.4-6 ng/mL (QC tested).
Protocol
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