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Human Tau-441 / 2N4R Pre-formed Fibrils Protein, Tag Free

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  • 分子别名（Synonym）

    DDPAC,FTDP-17,MAPT,MSTD,MTBT1,Tau,PHF-tau,TAU

  • 表达区间及表达系统（Source）

    Human Tau-441 Pre-formed Fibrils, Tag Free (TAU-H5115) is expressed from E. coli cells. It contains AA Met 1 - Leu 441 (Accession # P10636-8).

    Predicted N-terminus: Met 1

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    Tau Structure

    This protein carries no "tag".

    The protein has a calculated MW of 45.8 kDa.

  • 偶联（Conjugate）

    Unconjugated

  • 应用说明（Application）

    THT, cell assay, drug screening and other in vitro experiment.

  • 内毒素（Endotoxin）

    Less than 1.0 EU per μg by the LAL method.

  • 纯度（Purity）

    90% as determined by SDS-PAGE.

  • 制剂（Formulation）

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 运输（Shipping）

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储（Storage）

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product should be stored at -70°C or room temperature for short storage. Do not store fibrils on ice or at 4°C;
    2. The unsonicated fibril is validated to be stable after storage at -70°C for 1 year under sterile conditions;
    3. The sonicated fibril should be stored at -70°C for not more than 8 weeks.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

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  • 电泳（SDS-PAGE）

    Tau SDS-PAGE

    Tau-441 / 2N4R Pre-formed Fibrils monomer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • 活性（Bioactivity）-ThT Assay

    Tau THT

    Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time when tau wild-type monomers (Cat. No. TAU-H5117) are combined with tau wild-type Pre-formed Fibrils (Cat. No. TAU-H5115) (Routinely tested).

    Protocol

  • 电镜（Electron Microscope）

    Tau ELECTRON MICROSCOPE

    Transmission electron microscopy (TEM) of Tau-441 preformed fibrils (Cat. No. TAU-H5115). Fibril structure is visible on negative stain TEM images of TAU-H5115 (Routinely tested).

  • 活性（Bioactivity）-CELL BASE

    Tau CELL

    HEK293/Human Tau (GFP) Stable Cell Line (Cat. No. CHEK-ATP087) were transduced with Human Tau-441 Pre-formed Fibrils, Tag Free (Cat. No. TAU-H5115) and Human Tau-441, Tag Free (Cat. No. TAU-H5117) respectively. The fluorescence of GFP-Tau (Green) and DAPI (Blue) were detected by confocal microscope. A. Lipo2000 transduction. B. Lipo2000 and Human Tau-441, Tag Free transduction. C. Lipo2000 and Human Tau-441 Pre-formed Fibrils, Tag Free transduction. Scale bars, 50 μm (Routinely tested).

    Protocol

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背景介绍

Tau is a microtubule-associated protein, which encodes by the MAPT gene that located on chromosome 17q21. Tau Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. Hyperphosphorylation of the tau protein (tau inclusions, pTau) can result in the self-assembly of tangles of paired helical filaments and straight filaments, which are involved in the pathogenesis of Alzheimer's disease, frontotemporal dementia, and other tauopathies.

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药物研发进展
  • 英文全称：

    Microtubule-associated protein tau

  • 中文全称：

    微管相关蛋白tau

  • 种类：

  • 上市药物数量：

    1 详情

  • 临床药物数量：

    38 详情

  • 最高研发阶段：

    临床三期

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