Streptavidin Protein-Cy5

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STN-NC113-100ug
$80.00
In Stock - Delivery in 2-3 business days
STN-NC113-1mg (200ug X 5)
$165.00
In Stock - Delivery in 2-3 business days
STN-NC113
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Synonyms

Streptavidin, SA

Source

Streptavidin Protein-Cy5 (STN-NC113) is expressed from E. coli cells.

Molecular Characterization

This protein carries no "tag".

The protein has a calculated MW of 13.8 kDa.

Conjugate

Cy5

Excitation Wavelength: 651 nm

Emission Wavelength: 670 nm

Labeling

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with Cy5 using standard chemical labeling method. The residual Cy5 is removed by molecular sieve treatment during purification process.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

バックグランド

The Streptavidin Protein Europium chelate is a universal tool for TR-FRET assays that can bind biotinylated molecules. This product uses high-purity streptavidin (SA) covalently conjugated with Eu³⁺ chelate, and it can be used in combination with other Acceptors directly or indirectly labeled with fluorescent dyes. When the Donor and Acceptor come into close proximity (within a distance of less than 10 nm), a FRET reaction occurs: the 620 nm signal emitted by the Donor upon excitation by a specific light source is received by the Acceptor, which then emits a 665 nm signal.