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Streptavidin, SA
Streptavidin Protein-Alexa Fluor 647 (STN-NA119) is expressed from E. coli cells.
This protein carries no "tag".
The protein has a calculated MW of 13.8 kDa. The protein migrates as 12-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
The protein is designed as a tetramer.
AF647
Excitation Wavelength: 640 nm
Emission Wavelength: 672 nm
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF647 using standard chemical labeling method. The residual AF647 is removed by molecular sieve treatment during purification process.
The AF647 to protein molar ratio is 3-6.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.