user
menu
letter
Sign InRegister
langEnglisharrow

Streptavidin Protein-ALP, Alkaline Phosphatase conjugated Streptavidin

Cat. No. / Size
Price
Availability
Qty
STN-NA117-100ug
$65.00
In Stock - Delivery in 2-3 business days
STN-NA117-1mg
$175.00
In Stock - Delivery in 2-3 business days
STN-NA117
Sub-Total :
$ 0
Customize Product Request Quote
Contact Us
Contact Us
Sub-Total:
$ 0
Request Quote
Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

Streptavidin, SA

Source

Streptavidin is expressed from E. coli cells and conjugated with alkaline phosphatase under optimal conditions.

Molecular Characterization

This protein carries no "tag".

The protein has a calculated MW of 13.8 kDa.

Application

Recommended for use in ALP Chemiluminescence System such as CLEIA (0.1 µg/mL) or MPCLIA, immunohistochemistry, and western blot applications. Avoid using biotin-containing solutions as diluents. It is recommended that the reagent be titrated for optimal performance for each application. NOTE: Do not use skim milk as a blocking agent in the assay with streptavidin, since skim milk contains free biotin which will cause high backgrounds.

Formulation

Supplied as 0.2 μm filtered solution in 20 mM Tris, 3 M NaCl, pH7.5, 1% BSA, 0.03% Proclin300 with trehalose as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • The product MUST be stored at -70°C or lower upon receipt;
  • -70°C for 3 months under sterile conditions.
ACRO Quality Management System

Background

The Streptavidin Protein Terbium chelate is a universal tool for TR-FRET assays that can bind biotinylated molecules. This product uses high-purity streptavidin (SA) covalently conjugated with Tb³⁺ chelate, and it can be used in combination with other Acceptors directly or indirectly labeled with fluorescent dyes. When the Donor and Acceptor come into close proximity (within a distance of less than 10 nm), a FRET reaction occurs: the 620 nm signal emitted by the Donor upon excitation by a specific light source is received by the Acceptor, which then emits a 665 nm signal.