GMP Human Sonic Hedgehog / Shh (C24II) Protein

Sonic Hedgehog (SHH) is a morphogen protein that governs cell proliferation, patterning, and differentiation throughout the developing nervous system. During neural differentiation of stem cells, SHH signaling is indispensable for ventralization, specifying the identity of neural progenitor cells along the dorsal-ventral axis. It is particularly vital for generating ventral neuronal subtypes, including motor neurons from the spinal cord and midbrain dopaminergic neurons.

• Specific Neuronal Differentiation Driver​: Efficiently induces stem cell differentiation into neuronal progenitor cells and dopaminergic neurons.
• Safe Recombinant Expression​: Produced in an E. coli system to ensure high purity and enhanced safety profile.
• Long-Term Stability​: Maintains high activity stability with a shelf life of up to 5 years in lyophilized form at -20 °C to -70 °C.
• Cost-Effective Solution​: Achieves up to 50% cost reduction compared to conventional alternatives.

• Quality Assurance: Global QMS with comprehensive and stringent QC release criteria.
• End-to-End GMP Compliance: Full manufacturing and QC under a cGMP system.
• Comprehensive Control of Adventitious Agents: Stringent biosafety from cell banks to final release.
• Comprehensive Regulatory Support: Includes RSF and DMF to meet global requirements.
• Resilient Supply Chain: Intelligent modular facilities ensure a stable global supply.
• Professional Support: Extensive manufacturing and application expertise to accelerate development.

Met-Ile-Ile-Gly-Pro-Gly-Arg-Gly-Phe-Gly-Lys-Arg-Arg-His-Pro
(Routinely tested)

Less than 10 EU/mg, tested by the rFC method in compliance with USP <86> and Ph. Eur. 2.6.32.

<0.5 ng/µg of protein tested by ELISA.

<0.02 ng/μg of protein tested by qPCR.

Sterility testing was performed using the membrane filtration method in compliance with USP <71> and Ph. Eur. 2.6.1.

Negative

>95% as determined by SDS-PAGE.

Lyophilized from 0.22 μm filtered solution in PBS, 60 mM NaCl, pH7.4 with protectants.

Contact us for customized product form or formulation.

2R (13 mm neck finish)

This product is supplied and shipped on blue ice.

Upon receipt, store it immediately at -20°C or lower for long term storage.

Please avoid repeated freeze-thaw cycles.

• -20°C to -70°C for 5 years in lyophilized state;
• -70°C for 12 months under sterile conditions after reconstitution.