Human Semaphorin 3A / SEMA3A Protein, Fc Tag

分子别名（Synonym）

Semaphorin 3A,(semaphorin) 3A,coll-1,collapsin 1,Hsema-I,Hsema-III,MGC133243,sema domain,immunoglobulin domain (Ig),short basic domain,secreted,Sema III,SEMA1, Sema3A,SEMAD,SEMAIII,SEMAL,Semaphorin 3A,semaphorin D,Semaphorin III,semaphorin L,semaphorin-3A,SemD,SEMA3A

表达区间及表达系统（Source）

Human Semaphorin 3A, Fc Tag (SEA-H5257) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Val 771 (Accession # Q14563-1 (R552A, R555A)).

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 112.4 kDa. Besides Furin cleavage site (Arg 555 - Gln 556), the protein also has an unknown protease cleavage site at Leu 709 - Ile 710. The protein migrates as 100 kDa and 115 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

偶联（Conjugate）

Unconjugated

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程