Human NSE Protein, His Tag (MALS verified)

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NSE-H5144-25ug
$145.00
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NSE-H5144-100ug
$360.00
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NSE-H5144-500ug
$1,080.00
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NSE-H5144
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Synonyms

Neuron-specific enolase, gamma-enolase

Source

Human NSE, His Tag (NSE-H5144) is expressed from E. coli cells. It contains AA Met 1 - Leu 434 (Accession # P09104-1).

Predicted N-terminus: Met

Molecular Characterization

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 49.3 kDa. The protein migrates as 46-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

Neuron-specific enolase (NSE) is known to be a cell specific isoenzyme of the glycolytic enzyme enolase. It is also known as
gamma-enolase or nolase 2 (ENO2). It has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. NSE is a highly specific marker for neurons and peripheral neuroendocrine cells. In clinical, NSE could be used as a biomarker for neuronal injury.