user
menu
letter
Sign InRegister
langEnglisharrow
1/3
COA

Alexa Fluor™ 647-Labeled Human NCAM-1 / CD56 Protein, His TagStar Staining (HPLC verified)

1/3
COA
Cat. No. / Size
Price
Availability
Qty
NC1-HA2H4-25ug
$390.00
In Stock - Delivery in 2-3 business days
NC1-HA2H4-200ug
$2,480.00
In Stock - Delivery in 2 weeks
You're Invited to Join the In Vivo CAR Innovation Tools Co-Creation Program.
NC1-HA2H4
Sub-Total :
$ 0
Customize Product Request Quote
Contact Us
Contact Us
Sub-Total:
$ 0
Request Quote
Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

CD56, MSK39, NCAM1, N-CAM-1

Source

Alexa Fluor 647-Labeled Human NCAM-1 Protein, His Tag (NC1-HA2H4) is produced via conjugation of AF647 to Human NCAM-1, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human NCAM-1, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Gly 718 (Accession # P13591-2).

Predicted N-terminus: Leu 20

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 94.3 kDa. The protein migrates as 100-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Conjugate

AF647

Excitation Wavelength: 640 nm

Emission Wavelength: 672 nm

Protein Ratio

The AF647 to protein molar ratio is 0.5-1.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

NCAM1 belongs to the immunoglobulin superfamily of adhesion molecules. A wide range of alternatively spliced NCAM1 messenger RNAs (mRNAs) has been described to date, but only the 120-, 140-, and 180- kDa isoforms are commonly expressed. NCAM1 plays an important role in the regulation of neurogenesis, neurite outgrowth, proliferation, and cell migration, however, its function in hematopoiesis, including NK cells, is poorly understood. NCAM1 signaling is mediated either by homophilic or heterophilic interactions with fibroblast growth factor receptor (FGFR), L1-CAM, N-cadherin and other components of the extracellular matrix. Upon activation, NCAM1 triggers a variety of signaling cascades including FYN–focal adhesion kinase (FAK), MAPK, and phosphatidylinositol 3-kinase (PI3K) pathways.