Human HTT Protein, MBP,His Tag (MALS verified)

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HTT-H51M5-25ug
$105.00
In Stock - Delivery in 2-3 business days
HTT-H51M5-100ug
$270.00
In Stock - Delivery in 2-3 business days
HTT-H51M5-500ug
$805.00
In Stock - Delivery in 2-3 business days
HTT-H51M5
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Synonyms

HTT, Htt, Huntingtin

Source

Human HTT, MBP,His Tag (HTT-H51M5) is expressed from E. coli cells. It contains AA Met 1 - Glu 82 (Accession # P42858-1).

Predicted N-terminus: Met

Molecular Characterization

This protein carries a MBP tag at the N-terminus and a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 52.7 kDa. The protein migrates as 60-63 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

バックグランド

Huntingtin is the protein coded for by the HTT gene. Autosomal dominant mutation in the Huntingtin (Htt) protein is the cause of Huntington’s Disease (HD). In HD, the polyglutamine (polyQ) domain in the N-terminal sequenceof the protein is expanded beyond a threshold of 36 glutamines. mutant polyQ expansion strongly correlates in an inverse manner to disease age of onset.