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分子别名（Synonym）
Glypican 4,glypican proteoglycan 4,glypican-4,GPC4,K-glypican
表达区间及表达系统（Source）
Human Glypican 4, His Tag (GP4-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Ser 529 (Accession # O75487-1).
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 59.5 kDa. The protein migrates as 60-65 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
90% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human Glypican 4, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human Glypican 4, His Tag (Cat. No. GP4-H52H3) is more than 90% and the molecular weight of this protein is around 55-69 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) at 1 μg/mL (100 μL/well) can bind Human Glypican 4, His Tag (Cat. No. GP4-H52H3) with a linear range of 0.02-0.625 μg/mL (QC tested).
Protocol
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