user
menu
letter
Sign InRegister
langEnglisharrow

Human GAD2 / GAD65 Protein, His Tag

Cat. No. / Size
Price
Availability
Qty
GA2-H55H3-100ug
$360.00
In Stock - Delivery in 2-3 business days
GA2-H55H3-1mg
$2,380.00
In Stock - Delivery in 2-3 business days
GA2-H55H3
Sub-Total :
$ 0
Customize Product Request Quote
Contact Us
Contact Us
Sub-Total:
$ 0
Request Quote
Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

GAD2, GAD65, Glutamate decarboxylase 2

Source

Human GAD2 Protein, His Tag (GA2-H55H3) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Leu 585 (Accession # Q05329).

Predicted N-terminus: Met 1

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 67.3 kDa. The protein migrates as 65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with glycerol as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • The product MUST be stored at -70°C or lower upon receipt;
  • -70°C for 3 months under sterile conditions.
ACRO Quality Management System

Background

Glutamate decarboxylase or glutamic acid decarboxylase (GAD) is an enzyme that catalyzes the decarboxylation of glutamate to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. GAD uses pyridoxal-phosphate (PLP) as a cofactor.Two molecular forms of GAD (65 kDa and 67 kDa) are highly conserved and both are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice. GAD65 is an ampiphilic, membrane-anchored protein (585 a.a.), and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), and seems to be responsible for significant cytoplasmic GABA production.