Alexa Fluor™ 488-Labeled Human CD5 Protein, Fc Tag

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CD5-HA253-25ug
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CD5-HA253-200ug
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CD5-HA253
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Synonyms

CD5, LEU1

Source

Alexa Fluor 488-Labeled Human CD5 Protein, Fc Tag (CD5-HA253) is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Pro 372 (Accession # P06127-1). It is the Alexa Fluor™ 488 labeled form of Human CD5, Fc Tag (Cat. No. CD5-H5253).

Predicted N-terminus: Arg 25

Molecular Characterization

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 65.1 kDa.

Conjugate

AF488

Excitation Wavelength: 488 nm

Emission Wavelength: 517 nm

Labeling

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF488 using standard chemical labeling method. The residual AF488 is removed by molecular sieve treatment during purification process.

Protein Ratio

The AF488 to protein molar ratio is 2.1-3.4.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

CD5 protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes, and a subset of B lymphocytes. It serves to mitigate activating signals from the BCR, allowing B-1 cells to be activated only by strong stimuli. Additionally, CD5 is involved in regulating lipid synthesis and inflammatory responses.