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分子别名（Synonym）
CD70,CD27LG,TNFSF7,TNFSF7G,CD27L
表达区间及表达系统（Source）
PE-Labeled Human CD27 Ligand Protein, His Tag (CDL-HP246) is produced via conjugation of PE to Human CD27 Ligand Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human CD27 Ligand Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 52 - Pro 193 (Accession # P32970-1).
Predicted N-terminus: His
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 61.6 kDa.
偶联（Conjugate）
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
CAR阳性表达率检测（Evaluation of CAR expression）
FACS Analysis of Anti-CD27 Ligand CAR Expression
5e5 of anti-CD27 Ligand CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human CD27 Ligand Protein, His Tag (Cat. No.CDL-HP246) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). PE signal was used to evaluate the binding activity (QC tested).
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